The Columbus Crew have reportedly waived Romain Gall and Sergio Campbell, while Sporting KC and Orlando City each took the field in preseason action.
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Two MLS teams have made international additions, while the LA Galaxy and Philadelphia Union have revealed new kits ahead of the 2016 season.
The Chicago Fire have added to their backline with the addition of a Brazilian youth international, while a handful of MLS teams competed in preseason friendlies on Wednesday.
D.C. United acquired 34-year-old midfielder Marcelo Sarvas from the Colorado Rapids, while Venezuelan striker Fernando Aristeguieta is reportedly leaving the Philadelphia Union.
While teams have started their preseason schedules, the contract dispute between Kei Kamara and the Columbus Crew has heated up.
Eredivisie club FC Groningen confirmed it had transferred 25-year-old Johan Kappelhof to the Chicago Fire, while the Columbus Crew reportedly made a bid for Panamanian striker Ricardo Clarke.