At least two amateur teams will move on to take on MLS opponents in the fourth round.
All U.S. Open Cup Posts
Several amateur sides had plenty of fun at the expense of the USL.
The USL joins the Open Cup for the second round of the competition.
The second round of the U.S. Open Cup is set as the amateurs are ready to take on the pros.
Christos FC will not make another Open Cup run after falling to Reading United in the first round.
50 amatuer clubs from across the nation are in action today for the first round of the U.S. Open Cup.
Two of the three former NASL sides in the U.S. Open Cup managed to advance past the Play-In Round on Sunday night.